PureStem™ endothelial progenitor 30-SM2-1

This clonal human endothelial progenitor line was derived from the registered parental hES cell line ESI-017. Derivation was performed using a modified protocol for EB differentiation, where differentiation medium was supplemented in a step-wise fashion, with BMP4, activin A, FGF2, VEGF-A and the TGFb inhibitor - SB431542. This line expresses vascular endothelium cell surface markers, such as, TEK, PECAM1, CD34, CDH5, KDR, as well as both venous and arterial markers, indicating immature endothelial fate, such as, the arterial markers EFNB2, EPHB2 and venous/lymphatic markers, such as, LYVE1, EPHB4, NRP2 and SOX18.