The clonal human endothelial progenitor line was derived from the registered parental hES cell line ESI-017. Derivation was performed using a modified protocol for EB differentiation with differentiation medium supplemented, in a step-wise fashion, with BMP4, activin A, FGF2, VEGF-A and the TGFb inhibitor - SB431542. This line expresses vascular endothelium cell surface markers such as, vWF, CDH5, TEK, PECAM1, CD34, KDR, as well as specific venous/lympatic endothelial cell markers, such as, LYVE1 and SOX18.
EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
