The clonal human endothelial progenitor line 03 was derived from the registered parental hES cell line ESI-017. Derivation was achieved using a modified protocol for EB differentiation, where differentiation medium was supplemented, in a step-wise fashion, with BMP4, activin A, FGF2, VEGF-A and the TGFb inhibitor - SB431542. This line expresses vascular endothelium cell surface markers, such as, vWF, TEK, PECAM1, CD34, CDH5, KDR, as well as early endothelial markers, such as, TAL1, ETV2 and GATA2. This line expresses both venous and arterial markers, indicating immature endothelial fate, such as, the arterial markers EFNB2, NRP1 and the venous/lymphatic markers LYVE1, NRP2 and SOX18. This line also expresses the endodermal marker Sox17.
