EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
Content
All in Vitro Cells > Fetal Stem / Progenitor Cell card

Neural stem cells (University of Milan)

Lewis X-positive cells (LeX+) were isolated by immunomagnetic selection of cells from the spinal cords of E10.5 mice and expanded in vitro.


Grow LeX cells in Neurobasal medium (Invitrogen), containing B-27, N2, epidermal growth factor (20 ng/ml), fibroblast growth factor (FGF; 20 ng/ml), penicillin (100 U) and streptomycin (100g/ml). Grow cells as an adherent monolayer, and passage cultures every 5–7 d. Cells used for antigen selection should be passaged three to five times.
See additional Stem, Progenitor & Primary Cells for: Spinal Cord
Fetal Stem / Progenitor Cell
Mus musculus
Neural stem cells