Browser Not Supported.

IE users: please get the latest browser version at Microsoft Internet Explorer Home Page

if yot already have the latest browser version please verify that your browser settings is not in compatibility mode

Browser: unknown
Version: 0

EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
Content
All in Vitro Cells > Fetal Stem / Progenitor Cell card

Neural stem cells (University of Milan)

Lewis X-positive cells (LeX+) were isolated by immunomagnetic selection of cells from the spinal cords of E10.5 mice and expanded in vitro.


Grow LeX cells in Neurobasal medium (Invitrogen), containing B-27, N2, epidermal growth factor (20 ng/ml), fibroblast growth factor (FGF; 20 ng/ml), penicillin (100 U) and streptomycin (100g/ml). Grow cells as an adherent monolayer, and passage cultures every 5–7 d. Cells used for antigen selection should be passaged three to five times.
See additional Stem, Progenitor & Primary Cells for: Spinal Cord
Fetal Stem / Progenitor Cell
Mus musculus
Neural stem cells