Dental pulp stem cells (Nagoya University School of Medicine)

Normal adult third molar teeth were collected. Tooth surfaces were cleaned and cut around the cementum-enamel junction to reveal and isolate the pulp tissue. The tissue was then digested in a solution of collagenase type I (3 mg/ml) and dispase (4 mg/ml) to generate a single-cell suspension. Dental pulp single-cell suspensions were seeded onto tissue culture plates and cultured in alpha modification of Eagle's medium supplemented with FCS (20%), L-ascorbic acid 2-phosphate (100 μM),  L-glutamine (2 mM),  penicillin (100 units/ml), and streptomycin (100 μg/ml).