Stem cells from human exfoliated deciduous teeth (NIDCR)

Pulp tissue of normal exfoliated human deciduous incisors was digested in a solution of  collagenase type I (3 mg/ml) and dispase (4 mg/ml) for 1 hour. Single-cell suspensions were seeded on tissue culture plates and cultured in minimal essential medium, α-modification (α-MEM) supplemented with  FCS (20%),  L-ascorbic acid 2-phosphate (100 μM),  L-glutamine (2 mM),  penicillin (100 units/ml), and streptomycin (100 μg/ml).