Stem cells from human exfoliated deciduous teeth (The Fourth Military Medical University)

Pulp tissue was digested in a solution of type I collagenase (0.3 mg/mL) and dispase II (0.1%) for 1 hour. Single-cell suspensions were seeded onto tissue culture plates and cultured in Dulbecco's modified Eagle medium (DMEM) supplemented with fetal bovine serum (FBS, 15%), penicillin (100 units/mL), and streptomycin (100 mg/mL).