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Welcome to
LifeMap Discovery

Watch the introductory video to learn more about our database of embryonic development for stem cell research and regenerative medicine

Generation of adipocytes from mouse embryonic stem cells

This protocol describes a method to generate adipocyte-like cells, following an embryoid bodies formation step. The protocol yield lies between 50-70%. The differentiated cells contain lipid droplets and  express LPL, and FABP4 adipocyte-specific markers. The gene expression data for the embryoid bodies cells, was derived from a protocol that uses CD2341 (a retinoic acid receptor agonist), instead of retinoic acid.
21 days
See additional Protocols for: Adipose
Generation of adipocytes from mouse embryonic stem cells