Browser Not Supported.

IE users: please get the latest browser version at Microsoft Internet Explorer Home Page

if yot already have the latest browser version please verify that your browser settings is not in compatibility mode

Browser: unknown
Version: 0

EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
Content

Welcome to
LifeMap Discovery

Watch the introductory video to learn more about our database of embryonic development for stem cell research and regenerative medicine

Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells

This 4-step protocol describes a method for differentiation of brain microvascular endothelial cells (BMECs) from human embryonic stem cells (hESCs) by inducing simultaneous endothelial and neural cell differentiation, followed by purification of the BMECs on a selective matrix. An additional step of co-culturing with astrocyte cells can be performed for further estimation of the blood-brain barrier (BBB) properties of the differentiated cells. The BMECs express BBB markers, respond to astrocytic cues, and have barrier and transport properties similar to those of primary BMECs.
10-16 days
See additional Protocols for: Brain , Brain Barriers
Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells