Browser Not Supported.
IE users: please get the latest browser version at Microsoft Internet Explorer Home Page
if yot already have the latest browser version please verify that your browser settings is not in compatibility mode
Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells
This 4-step protocol describes a method for differentiation of brain microvascular endothelial cells (BMECs) from human embryonic stem cells (hESCs) by inducing simultaneous endothelial and neural cell differentiation, followed by purification of the BMECs on a selective matrix. An additional step of co-culturing with astrocyte cells can be performed for further estimation of the blood-brain barrier (BBB) properties of the differentiated cells. The BMECs express BBB markers, respond to astrocytic cues, and have barrier and transport properties similar to those of primary BMECs.