Browser Not Supported.
IE users: please get the latest browser version at Microsoft Internet Explorer Home Page
if yot already have the latest browser version please verify that your browser settings is not in compatibility mode
Generation of oligodendrocytes from epiblast stem cells
This protocol describes a method to generate oligodendrocytes from epiblast stem cells (EpiSC) via the formation of neural rosettes and oligodendrocyte progenitor cells (OPCs). In addition, it includes an expansion step that enables generation of high quantities of OPCs, for transplantation processes.
The generated oligodendrocytes and OPCs express specific markers, and have the capacity to produced myelin, as demonstrates by in-vitro culturing of OPCs with neurons, and by injection of OPCs into coronal, forebrain slices of early postnatal shiverer pups. Moreover EpibSC-derived OPCs formed patches of MBP+ myelin sheaths upon injection into the future corpus callosum of newborn, immunocompetent shiverer pups.