Generation of corneal epithelial cells using limbal fibroblast-conditioned medium
This protocol describes a method for generation of corneal epithelial cells from H1 and NCL1 human embryonic stem cell lines using limbal epithelial fibroblast-conditioned medium. The differentiated corneal cells express high levels of TP63 and KRT12 after 7-9 days of differentiation.
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Generation of corneal epithelial cells using limbal fibroblast-conditioned medium