Browser Not Supported.

IE users: please get the latest browser version at Microsoft Internet Explorer Home Page

if yot already have the latest browser version please verify that your browser settings is not in compatibility mode

Browser: unknown
Version: 0

EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
Content

Welcome to
LifeMap Discovery

Watch the introductory video to learn more about our database of embryonic development for stem cell research and regenerative medicine

Efficient derivation of purified lung and thyroid progenitor cells from embryonic stem cells

This protocol demonstrates purification and directed differentiation of lung and thyroid progenitor cells from mouse embryonic stem cells (ESCs). Inhibition of TGFb and BMP signaling, followed by combinatorial stimulation of BMP and FGF signaling, can distinctly differentiate these cells from definitive endodermal precursors. When derived using Nkx2-1::GFP knockin reporter ESCs, these progenitor cells can be purified and expanded in culture.  They feature a transcriptome that overlaps with developing lung epithelium. Upon induction, they can express a broad repertoire of markers indicative of lung and thyroid lineages and can recellularize a 3D lung tissue scaffold.

25 days
See additional Protocols for: Endoderm , Lung , Thyroid
Efficient derivation of purified lung and thyroid progenitor cells from embryonic stem cells