Generation of pancreatic progenitor cells using primary mesenchymal cells for expansion

In this protocol, primary embryonic mesenchymal cells are used as feeders for the expansion of definitive endoderm and endocrine progenitor cells. Mesenchymal progenitor cells can promote up to 6-million-fold expansion of embryonic stem cell-derived definitive endoderm cells. These expanded cells are further differentiated into pancreatic progenitor cells.