Derivation of embryonic germ cells and male gametes from mouse embryonic stem cells
This is a combined protocol for the generation of embryonic germ cells (EGCs) or male gametes from embryoid bodies (EBs) constructed from mouse embryonic stem cells (mESCs). The induced primordial germ cells mature into haploid male gametes that can be injected into oocytes and develop into blastocysts.
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Derivation of embryonic germ cells and male gametes from mouse embryonic stem cells