Generation of intermediate mesoderm cells from human pluripotent stem cells
This protocol describes a method to generate intermediate mesoderm (IM) cells, which can be sorted and induced to further differentiate into various cells of IM-derived lineages. This highly efficient protocol (more than 90% of the IM cells express OSR1) is based on 2D-differentiation of single embryonic stem cell cultures in serum-free medium.
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Generation of intermediate mesoderm cells from human pluripotent stem cells