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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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LifeMap Discovery

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Functional, immature hepatocyte differentiation from human embryonic stem cells

This protocol presents a means of inducing hESC to differentiate to hepatocyte-like cells, that limits cell proliferation without affecting cell viability. In the process of differentiation, cells take on a cuboidal/hexagonal-like structure morphology, and organize as a single layer. This differentiation strategy gives rise to morphologically and functionally hepatocyte-like cells which maintain immaturity (markers: Alf, Sox2).

28 days
See additional Protocols for: Liver
Functional, immature hepatocyte differentiation from human embryonic stem cells