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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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Derivation of lung epithelial cells from human embryonic stem cells via an air-liquid interface

This is the first human protocol to involve differentiation on filters in an air-liquid interface. The protocol is performed on 5 hESC lines (derived from preimplantation genetic diagnosed embryos; 4 lines have genetic mutations, unrelated to lung development).
28 days
See additional Protocols for: Lung
Derivation of lung epithelial cells from human embryonic stem cells via an air-liquid interface