Browser Not Supported.
IE users: please get the latest browser version at Microsoft Internet Explorer Home Page
if yot already have the latest browser version please verify that your browser settings is not in compatibility mode
Generation of motoneurons from stem cell by co-culture with MS5 cells in a defined medium
This 4-step protocol allows for the generation of motoneurons from human embryonic stem cell (hESC), by co-culture with MS5 stromal cells and then exposure to retinoic acid (RA) and sonic hedgehog (SHH). The derived motoneurons are functional and successfully integrate and outgrow when transplanted into the spinal cord of a chick embryo or an adult rat.