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EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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Motor neurons suspension-based differentiation of human embryonic stem cells

In this differentiation protocol, human embryonic stem cells are differentiated into motor neuron progenitor cells via a suspension-based procedure and the formation of neurospheres. The differentiated cells express specific motor neurons markers, such as MNX1 (HB9), Isl1, beta-III-tubulin, and ChAT (in long-term cultures). In addition, a conditioned medium, produced from the motor neurons cultures, contains neurotrophic factors such as nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), vascular endothelial growth factor (VEGF), neurotrophin 3 (NT-3), and neurotrophin 4 (NT-4). To investigate their functional properties, the differentiated cells were transplanted into rodent models of spinal cord injury (SCI), spinal muscular atrophy (SMA), and amyotrophic lateral sclerosis (ALS).
28 days
See additional Protocols for: Motor Neurons , Spinal Cord
Motor neurons suspension-based differentiation of human embryonic stem cells