Adipose-derived stem cells (Inst. Investigación Hospital Universitario La Paz)

Cells were obtained from a lipoaspirate from a female donor patient. The lipoaspirate was extensively washed with PBS, digested, and processed. The pellet was cultured in a noninductive medium consisting of DMEM containing sodium pyruvate (1 mM), glutamine (2 mM), fetal bovine serum (10%), and a penicillin G and streptomycin solution (100 U/ml). A total lipoaspirate of 400 ml yielded a primary culture of 1.5x10⁷ adherent cells. A confluence of 80%–90% was reached after 1 week of culture.