Mesenchymal stem cells were isolated from human amnion tissue of the placenta. The placentas were obtained from clinically normal pregnancies, at gestational age, 34-41 weeks. Cultured cell have adipogenic, chondrogenic, and osteogenic differentiation potential.
The amnion was mechanically peeled from the placenta, washed several times with phosphate-buffered saline (PBS), to remove excess blood, and incubated with type I collagenase for three hours, after being cut into small pieces. The mononuclear cells were collected by centrifugation. Cell pellets were washed and resuspended in α minimum essential medium (αMEM), supplemented with fetal bovine serum (10%), and basic fibroblast growth factor (bFGF, 10 ng/ml ). The cells were seeded into a T75 tissue culture flask. After 3-5 days of culturing, the small digested residues were removed, and the adherent cells were cultured.