Bone marrow-derived hematopoietic stem cells (BM-HSCs) are progenitor cells that can repopulate the bone marrow and differentiate into all blood cell types.
BM-HSCs were mobilized into the peripheral blood and collected by leukapheresis. Mobilization was achieved by administering granulocyte colony-stimulating factor (G-CSF) and cyclophosphamide to patients. Leukapheresis was performed using a commercially available apheresis devices, and cells expressing CD34 were selected. Cells were cryopreserved in liquid nitrogen for up to two weeks before treatment.