Bone marrow-derived mononuclear stem cells (BM-MNCs) are progenitor cells residing in the bone marrow. They have been shown to induce therapeutic angiogenesis.
450–500 ml bone marrow was aspirated from the iliac crest and anticoagulated with unfractionated 100 U/ml heparin. Cells were separated by Ficoll-Paque density gradient centrifugation. The resulting BM-MNC-rich pellet was resuspended and washed three times in isotonic saline. The pellet was resuspended in Ringer’s lactate solution to a final volume of 55–85 ml.