Bone marrow-derived mononuclear cells (BM-MNCs) are comprised of several cellular fractions, including: CD 34+ fraction (~1.5%), CD 45+ (~74.6%), CD 14+ (~8.4) and CD 34+ CD38- (~0.7%).
Bone marrow was collected by aspiration puncture in the anterosuperior iliac crest. Mononuclear stem cells were isolated by centrifugation using Ficoll-Hypaque density gradient medium (1.077 g/ml). The mononuclear fraction was then collected, washed in a heparinized saline solution containing 5% autologous serum and ﬁltered in order to remove the cellular adjunctive. The cells were again suspended in saline solution with autologous serum (5%), to make up an intramyocardial injection volume of 5ml.