Bone marrow-derived mononuclear cells (Instituto de Cardiologia do Rio Grande do Sul)

Bone marrow was collected by aspiration puncture in the anterosuperior iliac crest. Mononuclear stem cells were isolated by centrifugation using Ficoll-Hypaque density gradient medium (1.077 g/ml). The mononuclear fraction was then collected, washed in a heparinized saline solution containing 5% autologous serum and filtered in order to remove the cellular adjunctive. The cells were again suspended in saline solution with autologous serum (5%), to make up an intramyocardial injection volume of 5ml.