Peripheral blood-derived hematopoietic stem cells (Shanghai Jiao Tong University School of Medicine)

Hematopoietic stem cells were mobilized with cyclophosphamide (2g/m²) and granulocyte colony stimulating factor (10 µg/kg per day) and then collected from peripheral blood by leukapheresis using a continuous-flow blood cell separator when the CD34+ cells reached 10 cells/μL. Cells were frozen in dimethyl sulfoxide (10%), in a rate-controlled freezer, and stored in the vapor phase of liquid nitrogen.