Umbilical cord blood-derived mesenchymal stem cells (Vietnam National University, Ho Chi Minh City)

Umbilical cord blood mesenchymal stem cells (UCB-MSCs) were derived from cord blood (CB) collected from the umbilical cord vein.  CB was centrifuged at 2000 rpm for 15 minutes and the pellet was diluted at a 1:1 ratio with phosphate-buffered saline (PBS) and subjected to density centrifugation using Ficoll Hypaque (1.077 g/mL). Mononuclear cells (MNC) were isolated from the interphase and cultured in Iscove modified Dulbecco medium (IMDM) supplemented with activated platelet-rich plasma (aPRP, 10%) or fetal bovine serum (FBS, 10%), antibiotic-antimycotic (1%), epidermal growth factor (EGF, 10 ng/mL) and basic fibroblast growth factor (bFGF, 10 ng/mL). Media was replaced every 4 days.