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Umbilical cord blood-derived hematopoietic progenitor cells (Ducord) (Duke University)

Ducord consists of hematopoietic progenitor cells, monocytes, lymphocytes, and granulocytes, derived from human cord blood, intended for intravenous infusion. Blood recovered from umbilical cord and placenta is volume-reduced and partially depleted of red blood cells and plasma.

40 mL of cord blood is collected and a minimum of  109 nucleated cells is required to continue to processing. Red blood cell (RBC)-depletion and plasma reduction by an automated cell processor is performed. Hydroxyethyl starch (HES), at a 1:5 ratio, is added before centrifugation to assist with the separation of the RBCs. After separation of the components, approximately 21 mL of buffy coat–enriched product remains. Dimethyl sulfoxide/dextran (10% dimethyl sulfoxide in 5% dextran 40) is added as a cryoprotectant and the product is cryopreserved by controlled-rate freezing in a 25-mL double-compartment cryopreservation bag under liquid nitrogen.

See additional Stem, Progenitor & Primary Cells for: Blood , Umbilical Cord
Fetal Stem / Progenitor Cell
Homo sapiens
Umbilical cord blood-derived hematopoietic progenitor cells (Ducord)