Umbilical cord blood-derived hematopoietic stem cells (Universidade de São Paulo)

Cells were cultured under serum-free conditions, in FreeStyle 293 Expression Medium, supplemented with  human albumin (1%), flt-3 ligand (50 ng/mL ), thrombopoietin (35 ng/mL ), stem cell factor (50 ng/mL ), and  interleukin-6 (10 ng/mL).