Mesenchymal stem cells are derived from the bone marrow.
Bone marrow aspirate is resuspended in Dulbecco's phosphate-buffered saline (D-PBS) and processed by gradient density separation (Histopaque-1077) to isolate the fraction of mononuclear cells (MNCs). The resulting fraction is suspended in α-MEM containing fetal bovine serum (10%). Part of it is seeded and incubated in a CO2 incubator. Culture medium is changed every 3 days and cells in the developing adherent MSC layer are expanded by one or two successive passages.