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Bone marrow-derived mesenchymal stem cells (University of Cambridge)

Mesenchymal stem cells are derived from the bone marrow and isolated by adherence.

Bone-marrow mononuclear cells were separated by density gradient centrifugation in Ficoll-Paque™ PREMIUM. Washed cells were re-suspended in PBS/EDTA and cultured in Dulbecco's modified Eagle's low glucose medium supplemented with foetal bovine serum (10%), and plated at a density of 1 × 108 cells per cell-factory. Cultures were maintained in cell factories, at 37°C in a humidified atmosphere containing CO2 (5%). When the cultures were near confluence (>80%), cells were detached, by treatment with trypsin-EDTA (0.25%), and re-plated at 3.5 × 106 cells per cell factory. When 2 × 106 cells/kg patient body weight, or more, were obtained, MSCs were harvested and cryopreserved in human albumin solution (4.5%) with dimethyl sulphoxide (10%).

See additional Stem, Progenitor & Primary Cells for: Bone , Mesenchymal Stem Cells
Adult Stem / Progenitor Cell
Homo sapiens
Bone marrow-derived mesenchymal stem cells