All in Vitro Cells > Adult Stem / Progenitor Cell card

Neural stem cells LewisX+ CXCR4+ (University of Milan)

Neural stem cells are isolated from the subventricular zone of the adult brain of mice.

Single-cell suspensions are seeded at a density of 105 cells/ml in neurobasal medium, containing B27, N2, EGF (20 ng/ml), FGF (20 ng/ml), penicillin (100 U)/streptomycin (100 µg/ml). Cells are grown in uncoated T75 plastic flasks, as free-floating clusters (neurospheres). The cultures are passaged every 5–7 days. Cell isolation is performed by labeling with anti-LeX (SSEA-1) microbeads and purified by immunomagnetic selection. Positive cells are then selected for double positivity for LeX and CXCR4 by FACS.

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Adult Stem / Progenitor Cell
Mus musculus
Neural stem cells LewisX+ CXCR4+