Human endothelial progenitor cells (EPCs) were isolated from umbilical cord blood.
Mononuclear cord blood cells were harvested by a density gradient and plated in a cell culture dish with endothelial basal medium-2, changed daily for 2–3 weeks. An EPC colony with a typical cobblestone appearance developed after 2 weeks of seeding. The colony was removed by trypsinization and reseeded on gelatin-coated plates.