Limbal epithelial stem cells (University of Debrecen)

Limbal tissue was harvested from cadavers within 12 hours of biological death. The conjunctiva was incised and separated from the limbal junction. A small rectangular shape limbal graft was dissected away and towards the cornea. The graft was cultured on lens capsule cells in corneal epithelial cell culture medium, comprised of Dulbecco-modified Eagle’s medium (DMEM) supplemented with human AB serum (20%, human serum Type AB), L-glutamine ( 200 mM/mL),  penicillin (10,000 U/mL), and streptomycin (10 mg/mL).