All in Vitro Cells > Adult Stem / Progenitor Cell card

Hair follicle stem cells (Ludwig Maximilian University of Munich)

Hair follicle stem cells were isolated from mouse dorsal skin. Cells express various epithelial markers and have the capacity to differentiate into adipocytes, sebocytes and proteoglycan-producing cells.

Dorsal skin was removed and digested overnight, at 4°C, with dispase (2.5 mg/ml) in DMEM/F-12 medium.
The tissue was then subjected to a second digestion with
trypsin–EDTA (0.05%) for 5 min, washed, and plated onto tissue culture plates in DMEM/F12 (3:1) medium, supplemented with  fetal calf serum (FCS, 10%), B27 supplement (20 μl/ml), basic fibroblast growth factor  (bFGF, 20 ng/ml), and penicillin/streptomycin (1%).

See additional Stem, Progenitor & Primary Cells for: Epithelial Cells , Hair
Adult Stem / Progenitor Cell
Mus musculus
Hair follicle stem cells