Conjunctival fibroblasts (Yamaguchi University School of Medicine)

Two normal human conjunctivas were obtained upon informed consent of individuals undergoing eyelid or strabismus surgery, and were processed separately.
Each conjunctiva was incubated for 60 min at 37⁰C with dispase (2 mg/ml), and epithelial cells were mechanically removed. The remaining tissue was digested with collagenase (2 mg/ml), for 1 hr at 37⁰C. The cells from each conjunctiva were cultured separately in MEM supplemented with fetal bovine serum (10%).