Hair follicle stem cells (HFSC) are isolated from hair follicles of human scalp skin. HFSCs are CD200-positive and CD34-negative. Two subpopulations can be identified based on KERATIN 15 (KRT15) expression: KRT15-rich versus KRT15-poor cells. KRT15-rich cells are larger and yield fewer but wider colonies compared to KRT15-poor cells. The gene expression profile presented here relates to the general CD200-positive, CD34-negative population.
Hair follicles were isolated from human temporal scalps. Follicle cells were dissociated with trypsin (0.25%) containing ethylene diamine tetraacetic acid (EDTA, 0.03%) for 30 min, filtered through a cell strainer (40 μm pore) and cultured in defined keratinocyte serum-free medium (DKSFM).