Hair follicle stem cells (HFSCs) were isolated from 7, 15, 22, 28 and 32-day-old Sprague Dawley (SD) rats. Cells grow clonally, are slabstone-shaped and are HFS-, CD34-and integrin alpha 6-positive. When using a transwell system with dermal papilla (DP)-conditioned medium, cells differentiate into hair cells.
Hair follicle stem cells were isolated from hair follicles of 7, 15, 22, 28 and 32-day-old Sprague Dawley (SD) rats. The bulge region was dissociated with dispase (0.25%) for 30 minutes and then treated with trypsin (0.25%) for 15 minutes. Cell were maintained in Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 (DMEM/F12) medium.