This cell line was derived from human fetal neural stem (FNS) cells, that were isolated from the brain of a 9-week embryo. FNS cells were transfected with the plasmid vector pCAG2LMKOSimO carrying cDNA of the murine genes Oct4, Sox2, Klf4, and c-Myc. Cells express pluripotency markers and have the capacity to differentiate in-vitro to cells from the three embryonic germ layers.
Induced pluripotent stem cells are cultured in medium containing Knockout DMEM (88%), Knockout serum replacement (12%), glutamine/penicillin/streptomycin (X1), nonessential amino acids (X1), and basic fibroblasts growth factor (bFGF, 10 ng/ml). Cells are cultured on mitomycin-c-treated feeder cells derived from 12-day mouse embryos.