EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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All in Vitro Cells > Induced Pluripotent Stem Cell card

BC1 (Johns Hopkins University School of Medicine)

This induced pluripotent stem cell (iPSC) line was generated from CD34-positive bone marrow samples. Pluripotent cells were induced by a single EBNA1/OriP episomal plasmid expressing 5 reprogramming genes (OCT4, SOX2, KLF4, c-MYC and LIN28)  as a single poly-cistronic unit. Cells express stem cell markers and display normal pluripotency, as determined by embryoid body (EB) formation and in vitro differentiation. Cells have a normal karyotype 46, XY.


Cells are maintained on a mitotically inactivated mouse embryonic fibroblast (MEF) feeder layer in knockout/DMEM medium, supplemented with  knockout serum replacement (20%),  L-glutamine (2 mM), nonessential amino acids (2 mM), antibiotic/antimycotic mix (X1),  β-mercaptoethanol (0.1 mM), and  basic fibroblast growth factor (10 ng/ml).

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Induced Pluripotent Stem Cell
Homo sapiens
BC1