HSF-1 (UC01) (WiCell Research Institute, Inc.)

Cells are cultured on irradiated CF-1 mouse embryonic fibroblasts (MEFs) in hESC medium. Medium consists of knockout–Dulbecco’s modified Eagle’s medium (KO-DMEM), knockout serum replacer (KSR,20%), basic fibroblast growth factor (bFGF,4 μg/ml), nonessential amino acids (100 μM), β-mercaptoethanol (0.1 mM ), and glutamine (1 mM).