EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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All in Vitro Cells > Induced Pluripotent Stem Cell card

K04 (Harvard Stem Cell Institute)

This induced pluripotent stem cell (iPSC) line was generated from neonatal keratinocytes by infection of five factors, OCT4, SOX2, C-MYC, KLF4 and NANOG, using doxycycline (Dox)-inducible lentiviral vectors and selection of TRA-1-81-positive colonies. Karyotype: not determined.


Cells are cultured on a feeder layer of mitotically inactivated mouse embryonic fibroblasts plated on gelatin-coated tissue culture plates. Culture medium: Knockout (KO) DMEM, supplemented with KO-Serum Replacement (8% or 10%), Plasmanate (8% or 10%), fetal calf serum (5%), Glutamax-I (2 mM), non-essential amino acids (1%), penicillin (50 U/ml), streptomycin (50 ug/mL), beta-mercaptoethanol (0.055mM), recombinant hLIF (12ng/mL), and bFGF (5ng/mL).

See additional Stem, Progenitor & Primary Cells for: Inner Cell Mass
Induced Pluripotent Stem Cell
Homo sapiens
K04