EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
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All in Vitro Cells > Induced Pluripotent Stem Cell card

L1B41 (Osaka University Graduate School of Medicine)

The L1B41 induced pluripotent stem cell line was derived from corneal limbal epithelial cells by lentiviral transduction of OCT3/4, SOX2, C-MYC, and KLF4. Cells express pluripotency markers and form teratomas which contain cells from all the three germ layers. Cells have a normal karyotype, 46,XX.


Cells were seeded on MMC-treated mouse embryonic fibroblast (MEF) feeder cells and cultured in embryonic stem cell culture medium comprised of DMEM/F12,  supplemented with knockout serum replacement (20%), β-mercaptoethanol (0.1 mM), non-essential amino acid (0.1 mM), and bFGF (4 ng/ml). 

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Induced Pluripotent Stem Cell
Homo sapiens
L1B41