This induced pluripotent stem cell (iPSC) line was generated from MRC5 embryonic lung fibroblasts infected with a mix of moloney murine leukemia virus–derived vectors, each containing the coding sequences of one of the following human genes: OCT4, SOX2, c-Myc, or KLF4. Cells express pluripotency markers, and differentiate, in vitro, into cells of all the three germ layers.
For feeder- and serum-free culture, cells were grown in CDM, supplemented with activin (10 ng/ml) and bFGF (12 ng/ml). CDM: Iscove's modified Dulbecco's medium (50%) plus F12 NUT-MIX (50%), supplemented with insulin (7 μg/ml), transferrin (15 μg/ml), monothioglycerol (450 μM), and bovine serum albumin fraction V (5 mg/ml) or polyvinyl alcohol (CDM-PVA).
EMBRYONIC DEVELOPMENT & STEM CELL COMPENDIUM
