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Reprogrammed glycogen storage disease type 1a cells (University of Cambridge)

Induced pluripotent stem cells (iPSCs) were generated from fibroblasts derived from a 25-year-old male with glycogen storage disorder, caused by the absence of glucose-6-phosphate enzyme. The donated tissue was infected with a mix of moloney murine leukemia virus-derived vectors, each containing the coding sequences of one of the following human genes: OCT4, SOX2, c-MYC, or KLF4Cells express pluripotency markers, demonstrate alkaline phosphatase activity, and form teratomas which contain cells from all the three germ layers.

Cells were cultured on irradiated mouse feeder cells in human ES cell culture medium (KSR) supplemented with bFGF (4 ng/ml).

See additional Stem, Progenitor & Primary Cells for: Inner Cell Mass
Induced Pluripotent Stem Cell
Homo sapiens
Reprogrammed glycogen storage disease type 1a cells