Reprogrammed hereditary tyrosinemia type 1 cells (University of Cambridge)

Induced pluripotent stem cells (iPSCs) were generated from fibroblasts derived from a 2-month-old male with clinical features of hereditary tyrosinemia type 1, carrying a Val166Gly mutation in FAH gene (one allele). The donated tissue was infected with a mix of moloney murine leukemia virus-derived vectors, each containing the coding sequences of one of the following human genes: OCT4, SOX2, c-Myc, or KLF4. Cells express pluripotency markers, demonstrate alkaline phosphatase activity, and form teratomas, which contain cells from all the three germ layers.