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Reprogrammed hereditary tyrosinemia type 1 cells (University of Cambridge)

Induced pluripotent stem cells (iPSCs) were generated from fibroblasts derived from a 2-month-old male with clinical features of hereditary tyrosinemia type 1, carrying a Val166Gly mutation in FAH gene (one allele). The donated tissue was infected with a mix of moloney murine leukemia virus-derived vectors, each containing the coding sequences of one of the following human genes: OCT4, SOX2, c-Myc, or KLF4Cells express pluripotency markers, demonstrate alkaline phosphatase activity, and form teratomas, which contain cells from all the three germ layers.

Cells were cultured on irradiated mouse feeder cells in human ES cell culture medium (KSR) supplemented with bFGF (4 ng/ml).

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Induced Pluripotent Stem Cell
Homo sapiens
Reprogrammed hereditary tyrosinemia type 1 cells