Induced pluripotent stem cells (iPSCs) were derived from three maturity onset diabetes of the young type 2 (MODY2) patients and from five healthy donors. Cells carry a V62A mutation in the GCK gene. Cells were induced by retroviral transduction of OCT4, SOX2, KLF4, and c-MYC. Cells express pluripotency markers and can form teratomas in mice.
Induced pluripotent stem cells (iPSCs) were maintained on inactivated mouse embryonic fibroblasts (MEFs) in Dulbecco’s modified Eagle’s medium/Nutrient Mixture F-12 (DMEM/F12), supplemented with knockout serum replacement (KOSR, 20%), L-glutamine (2 mM), non-essential amino acids (NEAA, 10 mM), β-mercaptoethanol (50 mM), penicillin/ streptomycin (1X) and basic fibroblast growth factor (bFGF, 10 ng/ml).