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Rockefeller University embryonic stem cell line 2 (Rockefeller University)

This line was isolated from blastocysts that had been frozen on day six of in vitro development after in vitro fertilization. Karyotype analysis revealed that the line was female [46, XX], and had a normal karyotype.

These cells can be maintained on MEFs, by manual dissection, or transferred to and maintained on Matrigel-coated plates in MEF-conditioned medium. According to the provider's protocol, the growth medium should contains : DMEM (75%) supplemented with KSR (20%), GlutaMAX (2mM), MEM-NEAA (0.1mM), 2-mercaptoethanol (2mM), B27 supplement (without vitamin A), pen-strep (x1) and bFGF (20ng/ml).

See additional Stem, Progenitor & Primary Cells for: Inner Cell Mass
Embryonic Stem Cell
Homo sapiens
Rockefeller University embryonic stem cell line 2