The J1 ES line was derived from a male agouti 129S4/SvJae embryo. The J1 cells deposited at the ATCC were recloned from Dr. En Li's P6 J1 ES cells. The deposited line has been shown to be germline-competent.
Grow cells in HEPES-buffered (20 mM, pH 7.3) Dulbecco’s modified Eagle’s medium (DMEM) supplemented with fetal calf serum (15%), nonessential amino acids (0.1 mM), b-mercaptoethanol (0.1 mM), and antibiotics.
Grow cells on feeder layers of y-irradiated embryonic fibroblast (EF) cells.