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Reprogrammed sickle cell anemia cells (Whitehead Institute for Biomedical Research)

Induced pluripotent stem cells (iPSCs) were generated from fibroblast cultures of 12-week-old adult, male mice carrying the human mutant sickle gene (hβS/hβS). Cells were infected with retroviruses encoding for OCT4, SOX2, and KLF4 factors and a lentivirus encoding a 2-lox c-MYC cDNA. iPS cells were infected with an adenovirus encoding Cre recombinase to delete the lentivirus-transduced c-MYC. 

Cells were cultivated on irradiated mouse embryonic fibroblasts (MEFs) in DME medium containing FCS (15%), leukemia inhibiting factor (LIF), penicillin/streptomycin, L-glutamine, betamercaptoethanol and nonessential amino acids.

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Reprogrammed sickle cell anemia cells